sterilization and disinfection in microbiology pdf

Sterilization And Disinfection In Microbiology Pdf

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Last Updated on January 3, by Sagar Aryal. Microorganisms play an important role in causing infection and contamination. Therefore, Sterilization is an important technique in microbiology which helps to remove or destroy microorganisms from materials or surfaces.

Sterilisation and Disinfection: Methods and Principles | Microbiology

Let us make an in-depth study of the methods and basic principles of sterilisation and disinfection. Sterilisation can be effected by the following methods: I. Physical Methods and II. Chemical Methods. These two terms are used to describe the killing or removal of micro-organisms.

Sterilization refers to any process that removes, kills, or deactivates all forms of life in particular referring to microorganisms such as fungi , bacteria , spores , unicellular eukaryotic organisms such as Plasmodium , etc. Sterilization is distinct from disinfection , sanitization, and pasteurization , in that those methods reduce rather than eliminate all forms of life and biological agents present. After sterilization, an object is referred to as being sterile or aseptic. One of the first steps toward modernized sterilization was made by Nicolas Appert who discovered that thorough application of heat over a suitable period slowed the decay of foods and various liquids, preserving them for safe consumption for a longer time than was typical. Canning of foods is an extension of the same principle and has helped to reduce food borne illness "food poisoning". Other methods of sterilizing foods include food irradiation [3] [4] and high pressure pascalization.

Sterilization- physical and chemical methods

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Sterilization (microbiology)

If your institution subscribes to this resource, and you don't have a MyAccess Profile, please contact your library's reference desk for information on how to gain access to this resource from off-campus. Please consult the latest official manual style if you have any questions regarding the format accuracy. From the time of debates about the germ theory of disease, killing microbes before they reach patients has been a major strategy for preventing infection. In fact, Ignaz Semmelweis successfully applied disinfection principles decades before bacteria were first isolated.

Javascript seems to be disabled in your browser. You must have JavaScript enabled in your browser to utilize the functionality of this website. Sterilization can be achieved by a combination of heat, chemicals, irradiation, high pressure and filtration like steam under pressure, dry heat, ultraviolet radiation, gas vapor sterilants, chlorine dioxide gas etc. Effective sterilization techniques are essential for working in a lab and negligence of this could lead to severe consequences, it could even cost a life. So what are the most commonly used methods of sterilization in the laboratory, and how do they work?

This article is intended to provide a broad overview of common sterilization technologies and the basic elements of sterility assurance, followed by guidelines for selecting a sterilization process. A brief history of microbiology is provided to remind readers how recently it was discovered that sterility is a critical factor in medical procedures. Until the invention of the compound microscope, in which a second lens further magnifies the image from a first lens, individual microorganisms could not be observed. Simple lenses were not powerful enough to provide the magnification needed. Typical microorganisms vary between one half and several microns in size.

Sterilization and disinfection are the basic components of hospital infection control activities. Every day, a number of hospitals are performing various surgical procedures. Even more number of invasive procedures are being performed in different health care facilities.

1 comments

Jacob W.

The purpose of this Guidance Document for Disinfectants and Sterilization Methods is to assist lab personnel in their decisions involving the judicious selection and proper use of specific disinfectants and sterilization methods.

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